Gantlet of Conditions

Absiscic Acid (ABA)

ABA assays (2):

1. Vertical, Light, Root Length Assay

Germinate on base media for 2 d in cold, transfer to base media containing ABA (50µM) for 5d in the light.

2. Selection Assay

Germinate on base media containing ABA (15µM).

Media and Controls

Base Media: 0.5X MS, 1% Agar, S-, ABA preparation

Control(s): No ABA, solvent

Correlative Control(s): NaCl, PEG, Drought/Dessication