Gantlet of Conditions
ABA assays (2):
1. Vertical, Light, Root Length Assay
Germinate on base media for 2 d in cold, transfer to base media containing ABA (50µM) for 5d in the light.
2. Selection Assay
Germinate on base media containing ABA (15µM).
Base Media: 0.5X MS, 1% Agar, S-, ABA preparation
Control(s): No ABA, solvent
Correlative Control(s): NaCl, PEG, Drought/Dessication