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Gantlet of Conditions

Calcium

Calcium assay (1):

1. Vertical, Light, Root Length Assay

Recommended conditions for phenotypic analysis:

'Low Calcium' - regular MS media with 10 µM EGTA added

'High Calcium' - regular MS media (3 mM Ca), raise to 30 mM Ca with CaCl2

'High Calcium' - regular MS media (3 mM Ca), raise to 30 mM Ca with Ca(NO3)2

'High Calcium' - no MS salts, 30 mM Ca(NO3)2


Media and Controls

Base Media: Calcium preparation,

Control(s): 0.5X MS, 1% Agar, S-,

Correlative Control(s):


References

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