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Gantlet of Conditions

Ethidium Bromide (EtBr)

EtBr Assay:

1. Vertical, Dark, Hypocotyl Length Assay

Recommended condition to look for sensitive mutants: 25 µM EtBr. Recommended condition to look for resistant mutants: 100 µM EtBr.


Media and Controls

Base Media: 0.5X MS, 1% Agar, S-, EtBr preparation,

Contol(s): No EtBr, solvent,

Correlative controls:


References

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