Gantlet of Conditions

2010 Project

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Western Washington University

Arabidopsis Gantlet
Conditional Phenotype Analysis

Project Description

The purpose of this website is to publish protocols detailing a broad broad-spectrum panel of environmental and developmental assays that may be used to uncover conditional phenotypes in KO plants. To define each condition, we did the following:

1. identified environmental or chemical input,

2. referenced published dose response curves if available,

3. produced dose response curves for conditions not yet published,

- established quantifiable trait, i.e. root length,
- graphed for wild-type plants, ecotype Wassilevskija (Ws)
- identified threshold doses to test for sensitivity,
- identified saturation doses to test for insensitivity.

    Supported by the National Science Foundation.
    Supported by the National Science Foundation.